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MacBinary | 1990-06-10 | 8.5 KB | [ GCKc/GCK ]
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This file was processed as: MacBinary
(archive/macBinary ).
You can browse this item here: pGEM2
Confidence Program Detection Match Type Support
10%
dexvert
MacBinary (archive/macBinary)
fallback
Supported
10%
dexvert
Jesper Olsen Module (music/jesperOlsen)
magic
Supported
100%
file
MacBinary II, Wed Dec 13 11:30:40 1989, modified Sat Jun 9 21:11:48 1990, creator 'GCK ', type 'GCKc', 7984 bytes "pGEM2" , at 0x1fb0 480 bytes resource
default (weak)
99%
file
data
default
65%
TrID
TTComp archive compressed (bin-2K)
default (weak)
33%
TrID
MacBinary 2
default (weak)
0%
TrID
Sybase iAnywhere database files
default (weak)
100%
lsar
MacBinary
default
id metadata key value macFileType [ GCKc] macFileCreator [ GCK ]
hex view +--------+-------------------------+-------------------------+--------+--------+ |00000000| 00 05 70 47 45 4d 32 00 | 00 00 00 00 00 00 00 00 |..pGEM2.|........| |00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000040| 00 47 43 4b 63 47 43 4b | 20 00 00 00 00 00 00 00 |.GCKcGCK| .......| |00000050| 00 00 00 00 00 1f 30 00 | 00 01 e0 a1 ac 29 b0 a2 |......0.|.....)..| |00000060| 97 4e d4 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |.N......|........| |00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 c8 1c 00 00 |........|........| |00000080| 00 00 00 03 ff ff 80 01 | 00 00 00 00 00 00 00 00 |........|........| |00000090| 00 00 01 02 00 00 01 42 | 00 00 0b 39 00 00 0b 35 |.......B|...9...5| |000000a0| 47 41 41 54 41 43 41 43 | 47 47 41 41 54 54 43 47 |GAATACAC|GGAATTCG| |000000b0| 41 47 43 54 43 47 43 43 | 43 47 47 47 47 41 54 43 |AGCTCGCC|CGGGGATC| |000000c0| 43 54 43 54 41 47 41 47 | 54 43 47 41 43 43 54 47 |CTCTAGAG|TCGACCTG| |000000d0| 43 41 47 43 43 43 41 41 | 47 43 54 54 43 43 47 47 |CAGCCCAA|GCTTCCGG| |000000e0| 54 43 54 43 43 43 54 41 | 54 41 47 54 47 41 47 54 |TCTCCCTA|TAGTGAGT| |000000f0| 43 47 54 41 54 54 41 41 | 54 54 54 43 47 41 54 41 |CGTATTAA|TTTCGATA| |00000100| 41 47 43 43 41 47 43 54 | 47 43 41 54 54 41 41 54 |AGCCAGCT|GCATTAAT| |00000110| 47 41 41 54 43 47 47 43 | 43 41 41 43 47 43 47 43 |GAATCGGC|CAACGCGC| |00000120| 47 47 47 47 41 47 41 47 | 47 43 47 47 54 54 54 47 |GGGGAGAG|GCGGTTTG| |00000130| 43 47 54 41 54 54 47 47 | 47 43 47 43 54 43 54 54 |CGTATTGG|GCGCTCTT| |00000140| 43 43 47 43 54 54 43 43 | 54 43 47 43 54 43 41 43 |CCGCTTCC|TCGCTCAC| |00000150| 54 47 41 43 54 43 47 43 | 54 47 43 47 43 54 43 47 |TGACTCGC|TGCGCTCG| |00000160| 47 54 43 47 54 54 43 47 | 47 43 54 47 43 47 47 43 |GTCGTTCG|GCTGCGGC| |00000170| 47 41 47 43 47 47 54 41 | 54 43 41 47 43 54 43 41 |GAGCGGTA|TCAGCTCA| |00000180| 43 54 43 41 41 41 47 47 | 43 47 47 54 41 41 54 41 |CTCAAAGG|CGGTAATA| |00000190| 43 47 47 54 54 41 54 43 | 43 41 43 41 47 41 41 54 |CGGTTATC|CACAGAAT| |000001a0| 43 41 47 47 47 47 41 54 | 41 41 43 47 43 41 47 47 |CAGGGGAT|AACGCAGG| |000001b0| 41 41 41 47 41 41 43 41 | 54 47 54 47 41 47 43 41 |AAAGAACA|TGTGAGCA| |000001c0| 41 41 41 47 47 43 43 41 | 47 43 41 41 41 41 47 47 |AAAGGCCA|GCAAAAGG| |000001d0| 43 43 41 47 47 41 41 43 | 43 47 54 41 41 41 41 41 |CCAGGAAC|CGTAAAAA| |000001e0| 47 47 43 43 47 43 47 54 | 54 47 43 54 47 47 43 47 |GGCCGCGT|TGCTGGCG| |000001f0| 54 54 54 54 54 43 43 41 | 54 41 47 47 43 54 43 43 |TTTTTCCA|TAGGCTCC| |00000200| 47 43 43 43 43 43 43 54 | 47 41 43 47 41 47 43 41 |GCCCCCCT|GACGAGCA| |00000210| 54 43 41 43 41 41 41 41 | 41 54 43 47 41 43 47 43 |TCACAAAA|ATCGACGC| |00000220| 54 43 41 41 47 54 43 41 | 47 41 47 47 54 47 47 43 |TCAAGTCA|GAGGTGGC| |00000230| 47 41 41 41 43 43 43 47 | 41 43 41 47 47 41 43 54 |GAAACCCG|ACAGGACT| |00000240| 41 54 41 41 41 47 41 54 | 41 43 43 41 47 47 43 47 |ATAAAGAT|ACCAGGCG| |00000250| 54 54 54 43 43 43 43 43 | 54 47 47 41 41 47 43 54 |TTTCCCCC|TGGAAGCT| |00000260| 43 43 43 54 43 47 54 47 | 43 47 43 54 43 54 43 43 |CCCTCGTG|CGCTCTCC| |00000270| 54 47 54 54 43 43 47 41 | 43 43 43 54 47 43 43 47 |TGTTCCGA|CCCTGCCG| |00000280| 43 54 54 41 43 43 47 47 | 41 54 41 43 43 54 47 54 |CTTACCGG|ATACCTGT| |00000290| 43 43 47 43 43 54 54 54 | 43 54 43 43 43 54 54 43 |CCGCCTTT|CTCCCTTC| |000002a0| 47 47 47 41 41 47 43 47 | 54 47 47 43 47 43 54 54 |GGGAAGCG|TGGCGCTT| |000002b0| 54 43 54 43 41 54 41 47 | 43 54 43 41 43 47 43 54 |TCTCATAG|CTCACGCT| |000002c0| 47 54 41 47 47 54 41 54 | 43 54 43 41 47 54 54 43 |GTAGGTAT|CTCAGTTC| |000002d0| 47 47 54 47 54 41 47 47 | 54 43 47 54 54 43 47 43 |GGTGTAGG|TCGTTCGC| |000002e0| 54 43 43 41 41 47 43 54 | 47 47 47 43 54 47 54 47 |TCCAAGCT|GGGCTGTG| |000002f0| 54 47 43 41 43 47 41 41 | 43 43 43 43 43 43 47 54 |TGCACGAA|CCCCCCGT| |00000300| 54 43 41 47 43 43 43 47 | 41 43 43 47 43 54 47 43 |TCAGCCCG|ACCGCTGC| |00000310| 47 43 43 54 54 41 54 43 | 43 47 47 54 41 41 43 54 |GCCTTATC|CGGTAACT| |00000320| 41 54 43 47 54 43 54 54 | 47 41 47 54 43 43 41 41 |ATCGTCTT|GAGTCCAA| |00000330| 43 43 43 47 47 54 41 41 | 47 41 43 41 43 47 41 43 |CCCGGTAA|GACACGAC| |00000340| 54 54 41 54 43 47 43 43 | 41 43 54 47 47 43 41 47 |TTATCGCC|ACTGGCAG| |00000350| 43 41 47 43 43 41 43 54 | 47 47 54 41 41 43 41 47 |CAGCCACT|GGTAACAG| |00000360| 47 41 54 54 41 47 43 41 | 47 41 47 43 47 41 47 47 |GATTAGCA|GAGCGAGG| |00000370| 54 41 54 47 54 41 47 47 | 43 47 47 54 47 43 54 41 |TATGTAGG|CGGTGCTA| |00000380| 43 41 47 41 47 54 54 43 | 54 54 47 41 41 47 54 47 |CAGAGTTC|TTGAAGTG| |00000390| 47 54 47 47 43 43 54 41 | 41 43 54 41 43 47 47 43 |GTGGCCTA|ACTACGGC| |000003a0| 54 41 43 41 43 54 41 47 | 41 41 47 47 41 43 41 47 |TACACTAG|AAGGACAG| |000003b0| 54 41 54 54 54 47 47 54 | 41 54 43 54 47 43 47 43 |TATTTGGT|ATCTGCGC| |000003c0| 54 43 54 47 43 54 47 41 | 41 47 43 43 41 47 54 54 |TCTGCTGA|AGCCAGTT| |000003d0| 41 43 43 54 54 43 47 47 | 41 41 41 41 41 47 41 47 |ACCTTCGG|AAAAAGAG| |000003e0| 54 54 47 47 54 41 47 43 | 54 43 54 54 47 41 54 43 |TTGGTAGC|TCTTGATC| |000003f0| 43 47 47 43 41 41 41 43 | 41 41 41 43 43 41 43 43 |CGGCAAAC|AAACCACC| |00000400| 47 43 54 47 47 54 41 47 | 43 47 47 54 47 47 54 54 |GCTGGTAG|CGGTGGTT| |00000410| 54 54 54 54 54 47 54 54 | 54 47 43 41 41 47 43 41 |TTTTTGTT|TGCAAGCA| |00000420| 47 43 41 47 41 54 54 41 | 43 47 43 47 43 41 47 41 |GCAGATTA|CGCGCAGA| |00000430| 41 41 41 41 41 41 47 47 | 41 54 43 54 43 41 41 47 |AAAAAAGG|ATCTCAAG| |00000440| 41 41 47 41 54 43 43 54 | 54 54 47 41 54 43 54 54 |AAGATCCT|TTGATCTT| |00000450| 54 54 43 54 41 43 47 47 | 47 47 54 43 54 47 41 43 |TTCTACGG|GGTCTGAC| |00000460| 47 43 54 43 41 47 54 47 | 47 41 41 43 47 41 41 41 |GCTCAGTG|GAACGAAA| |00000470| 41 43 54 43 41 43 47 54 | 54 41 41 47 47 47 41 54 |ACTCACGT|TAAGGGAT| |00000480| 54 54 54 47 47 54 43 41 | 54 47 41 47 41 54 54 41 |TTTGGTCA|TGAGATTA| |00000490| 54 43 41 41 41 41 41 47 | 47 41 54 43 54 54 43 41 |TCAAAAAG|GATCTTCA| |000004a0| 43 43 54 41 47 41 54 43 | 43 54 54 54 54 41 41 41 |CCTAGATC|CTTTTAAA| |000004b0| 54 54 41 41 41 41 41 54 | 47 41 41 47 54 54 54 54 |TTAAAAAT|GAAGTTTT| |000004c0| 41 41 41 54 43 41 41 54 | 43 54 41 41 41 47 54 41 |AAATCAAT|CTAAAGTA| |000004d0| 54 41 54 41 54 47 41 47 | 54 41 41 41 43 54 54 47 |TATATGAG|TAAACTTG| |000004e0| 47 54 43 54 47 41 43 41 | 47 54 54 41 43 43 41 41 |GTCTGACA|GTTACCAA| |000004f0| 54 47 43 54 54 41 41 54 | 43 41 47 54 47 41 47 47 |TGCTTAAT|CAGTGAGG| |00000500| 43 41 43 43 54 41 54 43 | 54 43 41 47 43 47 41 54 |CACCTATC|TCAGCGAT| |00000510| 43 54 47 54 43 54 41 54 | 54 54 43 47 54 54 43 41 |CTGTCTAT|TTCGTTCA| |00000520| 54 43 43 41 54 41 47 54 | 54 47 43 43 54 47 41 43 |TCCATAGT|TGCCTGAC| |00000530| 54 43 43 43 43 47 54 43 | 47 54 47 54 41 47 41 54 |TCCCCGTC|GTGTAGAT| |00000540| 41 41 43 54 41 43 47 41 | 54 41 43 47 47 47 41 47 |AACTACGA|TACGGGAG| |00000550| 47 47 43 54 54 41 43 43 | 41 54 43 54 47 47 43 43 |GGCTTACC|ATCTGGCC| |00000560| 43 43 41 47 54 47 43 54 | 47 43 41 41 54 47 41 54 |CCAGTGCT|GCAATGAT| |00000570| 41 43 43 47 43 47 41 47 | 41 43 43 43 41 43 47 43 |ACCGCGAG|ACCCACGC| |00000580| 54 43 41 43 43 47 47 43 | 54 43 43 41 47 41 54 54 |TCACCGGC|TCCAGATT| |00000590| 54 41 54 43 41 47 43 41 | 41 54 41 41 41 43 43 41 |TATCAGCA|ATAAACCA| |000005a0| 47 43 43 41 47 43 43 47 | 47 41 41 47 47 47 43 43 |GCCAGCCG|GAAGGGCC| |000005b0| 47 41 47 43 47 43 41 47 | 41 41 47 54 47 47 54 43 |GAGCGCAG|AAGTGGTC| |000005c0| 43 54 47 43 41 41 43 54 | 54 54 41 54 43 43 47 43 |CTGCAACT|TTATCCGC| |000005d0| 43 54 43 43 41 54 43 43 | 41 47 54 43 54 41 54 54 |CTCCATCC|AGTCTATT| |000005e0| 41 41 54 54 47 54 54 47 | 43 43 47 47 47 41 41 47 |AATTGTTG|CCGGGAAG| |000005f0| 43 54 41 47 41 47 54 41 | 41 47 54 41 47 54 54 43 |CTAGAGTA|AGTAGTTC| |00000600| 47 43 43 41 47 54 54 41 | 41 54 41 47 54 54 54 47 |GCCAGTTA|ATAGTTTG| |00000610| 43 47 43 41 41 43 47 54 | 54 47 54 54 47 43 43 41 |CGCAACGT|TGTTGCCA| |00000620| 54 54 47 43 54 41 43 41 | 47 47 43 41 54 43 47 54 |TTGCTACA|GGCATCGT| |00000630| 47 47 54 47 54 43 41 43 | 47 43 54 43 47 54 43 47 |GGTGTCAC|GCTCGTCG| |00000640| 54 54 54 47 47 54 41 54 | 47 47 43 54 54 43 41 54 |TTTGGTAT|GGCTTCAT| |00000650| 54 43 41 47 43 54 43 43 | 47 47 54 54 43 43 43 41 |TCAGCTCC|GGTTCCCA| |00000660| 41 43 47 41 54 43 41 41 | 47 47 43 47 41 47 54 54 |ACGATCAA|GGCGAGTT| |00000670| 41 43 41 54 47 41 54 43 | 43 43 43 43 41 54 47 54 |ACATGATC|CCCCATGT| |00000680| 54 47 54 47 43 41 41 41 | 41 41 41 47 43 47 47 54 |TGTGCAAA|AAAGCGGT| |00000690| 54 41 47 43 54 43 43 54 | 54 43 47 47 54 43 43 54 |TAGCTCCT|TCGGTCCT| |000006a0| 43 43 47 41 54 43 47 54 | 54 47 54 43 41 47 41 41 |CCGATCGT|TGTCAGAA| |000006b0| 47 54 41 41 47 54 54 47 | 47 43 43 47 43 41 47 54 |GTAAGTTG|GCCGCAGT| |000006c0| 47 54 54 41 54 43 41 43 | 54 43 41 54 47 47 54 54 |GTTATCAC|TCATGGTT| |000006d0| 41 54 47 47 43 41 47 43 | 41 43 54 47 43 41 54 41 |ATGGCAGC|ACTGCATA| |000006e0| 41 54 54 43 54 43 54 54 | 41 43 54 47 54 43 41 54 |ATTCTCTT|ACTGTCAT| |000006f0| 47 43 43 41 54 43 43 47 | 54 41 41 47 41 54 47 43 |GCCATCCG|TAAGATGC| |00000700| 54 54 54 54 43 54 47 54 | 47 41 43 54 47 47 54 47 |TTTTCTGT|GACTGGTG| |00000710| 41 47 54 41 43 54 43 41 | 41 43 43 41 41 47 54 43 |AGTACTCA|ACCAAGTC| |00000720| 41 54 54 43 54 47 41 47 | 41 41 54 41 47 54 47 54 |ATTCTGAG|AATAGTGT| |00000730| 41 54 47 43 47 47 43 47 | 41 43 43 47 41 47 54 54 |ATGCGGCG|ACCGAGTT| |00000740| 47 43 54 43 54 54 47 43 | 43 43 47 47 43 47 54 43 |GCTCTTGC|CCGGCGTC| |00000750| 41 41 54 41 43 47 47 47 | 41 54 41 41 54 41 43 43 |AATACGGG|ATAATACC| |00000760| 47 43 47 43 43 41 43 41 | 54 41 47 43 41 47 41 41 |GCGCCACA|TAGCAGAA| |00000770| 43 54 54 54 41 41 41 41 | 47 54 47 43 54 43 41 54 |CTTTAAAA|GTGCTCAT| |00000780| 43 41 54 54 47 47 41 41 | 41 41 43 47 54 54 43 54 |CATTGGAA|AACGTTCT| |00000790| 54 43 47 47 47 47 43 47 | 41 41 41 41 43 54 43 54 |TCGGGGCG|AAAACTCT| |000007a0| 43 41 41 47 47 41 54 43 | 54 54 41 43 47 54 54 47 |CAAGGATC|TTACGTTG| |000007b0| 41 47 41 54 43 43 41 47 | 54 54 43 47 41 54 47 54 |AGATCCAG|TTCGATGT| |000007c0| 41 41 43 43 43 41 43 54 | 43 47 54 47 43 41 43 43 |AACCCACT|CGTGCACC| |000007d0| 43 41 41 43 54 47 41 54 | 43 54 54 43 41 47 43 41 |CAACTGAT|CTTCAGCA| |000007e0| 54 43 54 54 54 54 41 43 | 54 54 54 43 41 43 43 41 |TCTTTTAC|TTTCACCA| |000007f0| 47 43 47 54 54 54 43 54 | 47 47 47 54 47 41 47 43 |GCGTTTCT|GGGTGAGC| |00000800| 41 41 41 41 41 43 41 47 | 47 41 41 47 47 43 41 41 |AAAAACAG|GAAGGCAA| |00000810| 41 41 54 47 43 43 47 43 | 41 41 41 41 41 41 47 47 |AATGCCGC|AAAAAAGG| |00000820| 47 41 41 54 41 41 47 47 | 47 43 47 41 43 41 43 47 |GAATAAGG|GCGACACG| |00000830| 47 41 41 41 54 47 54 54 | 47 41 41 54 41 43 54 43 |GAAATGTT|GAATACTC| |00000840| 41 54 41 43 54 43 54 54 | 43 43 54 54 54 54 54 43 |ATACTCTT|CCTTTTTC| |00000850| 41 41 54 41 54 54 41 54 | 54 47 41 41 47 43 41 54 |AATATTAT|TGAAGCAT| |00000860| 54 54 41 54 43 41 47 47 | 47 54 54 41 54 54 47 54 |TTATCAGG|GTTATTGT| |00000870| 43 54 43 41 54 47 41 47 | 43 47 47 41 54 41 43 41 |CTCATGAG|CGGATACA| |00000880| 54 41 54 54 54 47 41 41 | 54 47 54 41 54 54 54 41 |TATTTGAA|TGTATTTA| |00000890| 47 41 41 41 41 41 54 41 | 41 41 43 41 41 41 54 41 |GAAAAATA|AACAAATA| |000008a0| 47 47 47 47 54 54 43 43 | 47 43 47 43 41 43 41 54 |GGGGTTCC|GCGCACAT| |000008b0| 54 54 43 43 43 43 47 41 | 41 41 41 47 54 47 43 43 |TTCCCCGA|AAAGTGCC| |000008c0| 41 43 43 54 47 41 43 47 | 54 43 54 41 41 47 41 41 |ACCTGACG|TCTAAGAA| |000008d0| 41 43 43 41 54 54 41 54 | 54 41 54 43 41 54 47 41 |ACCATTAT|TATCATGA| |000008e0| 43 41 54 54 41 41 43 43 | 54 41 54 41 41 41 41 41 |CATTAACC|TATAAAAA| |000008f0| 54 41 47 47 43 47 54 41 | 54 43 41 43 47 41 47 47 |TAGGCGTA|TCACGAGG| |00000900| 43 43 43 54 54 54 43 47 | 54 43 54 43 47 43 47 43 |CCCTTTCG|TCTCGCGC| |00000910| 47 54 54 54 43 47 47 54 | 47 41 54 47 41 43 47 47 |GTTTCGGT|GATGACGG| |00000920| 54 47 41 41 54 47 41 41 | 41 41 43 43 54 43 54 47 |TGAATGAA|AACCTCTG| |00000930| 41 43 41 43 41 54 47 43 | 41 47 43 54 43 43 43 47 |ACACATGC|AGCTCCCG| |00000940| 47 41 47 41 43 47 47 54 | 43 41 43 41 47 43 54 54 |GAGACGGT|CACAGCTT| |00000950| 47 54 43 54 47 54 41 41 | 47 43 47 47 41 54 47 43 |GTCTGTAA|GCGGATGC| |00000960| 43 47 47 47 41 47 43 41 | 47 41 43 41 41 47 43 43 |CGGGAGCA|GACAAGCC| |00000970| 43 47 54 43 41 47 47 47 | 43 47 43 47 54 43 41 47 |CGTCAGGG|CGCGTCAG| |00000980| 43 47 47 47 54 47 54 54 | 47 47 43 47 47 47 54 47 |CGGGTGTT|GGCGGGTG| |00000990| 54 43 47 47 47 47 43 54 | 47 47 43 54 54 41 41 43 |TCGGGGCT|GGCTTAAC| |000009a0| 54 41 54 47 43 47 47 43 | 41 54 43 41 47 41 47 43 |TATGCGGC|ATCAGAGC| |000009b0| 41 47 41 54 54 47 54 41 | 43 54 47 41 47 41 47 54 |AGATTGTA|CTGAGAGT| |000009c0| 47 43 41 43 43 41 54 41 | 54 43 47 41 43 47 43 54 |GCACCATA|TCGACGCT| |000009d0| 43 54 43 43 43 54 54 41 | 54 47 43 47 41 43 54 43 |CTCCCTTA|TGCGACTC| |000009e0| 43 54 47 43 41 54 54 41 | 47 47 41 41 47 43 41 47 |CTGCATTA|GGAAGCAG| |000009f0| 43 43 43 41 47 54 41 47 | 54 41 47 47 54 54 47 41 |CCCAGTAG|TAGGTTGA| |00000a00| 47 47 43 43 47 54 54 47 | 41 47 43 41 43 43 47 43 |GGCCGTTG|AGCACCGC| |00000a10| 43 47 43 43 47 43 41 41 | 47 47 41 41 54 47 47 54 |CGCCGCAA|GGAATGGT| |00000a20| 47 43 41 54 47 43 41 41 | 47 47 41 47 41 54 47 47 |GCATGCAA|GGAGATGG| |00000a30| 43 47 43 43 43 41 41 43 | 41 47 54 43 43 43 43 43 |CGCCCAAC|AGTCCCCC| |00000a40| 47 47 43 43 41 43 47 47 | 47 47 43 43 54 47 43 43 |GGCCACGG|GGCCTGCC| |00000a50| 41 43 43 41 54 41 43 43 | 43 41 43 47 43 43 47 41 |ACCATACC|CACGCCGA| |00000a60| 41 41 43 41 41 47 43 47 | 43 54 43 41 54 47 41 47 |AACAAGCG|CTCATGAG| |00000a70| 43 43 43 47 41 41 47 54 | 47 47 43 47 41 47 43 43 |CCCGAAGT|GGCGAGCC| |00000a80| 43 47 41 54 43 54 54 43 | 43 43 43 41 54 43 47 47 |CGATCTTC|CCCATCGG| |00000a90| 54 47 41 54 47 54 43 47 | 47 43 47 41 54 41 54 41 |TGATGTCG|GCGATATA| |00000aa0| 47 47 43 47 43 43 41 47 | 43 41 41 43 43 47 43 41 |GGCGCCAG|CAACCGCA| |00000ab0| 43 43 54 47 54 47 47 43 | 47 43 43 47 47 54 47 41 |CCTGTGGC|GCCGGTGA| |00000ac0| 54 47 43 43 47 47 43 43 | 41 43 47 41 54 47 43 47 |TGCCGGCC|ACGATGCG| |00000ad0| 54 43 43 47 47 43 47 54 | 41 47 41 47 47 41 54 43 |TCCGGCGT|AGAGGATC| |00000ae0| 54 47 47 43 54 41 47 43 | 47 41 54 47 41 43 43 43 |TGGCTAGC|GATGACCC| |00000af0| 54 47 43 54 47 41 54 54 | 47 47 54 54 43 47 43 54 |TGCTGATT|GGTTCGCT| |00000b00| 47 41 43 43 41 54 54 54 | 43 43 47 47 47 47 54 47 |GACCATTT|CCGGGGTG| |00000b10| 43 47 47 41 41 43 47 47 | 43 47 54 54 41 43 43 41 |CGGAACGG|CGTTACCA| |00000b20| 47 41 41 41 43 54 43 41 | 47 41 41 47 47 54 54 43 |GAAACTCA|GAAGGTTC| |00000b30| 47 54 43 43 41 41 43 43 | 41 41 41 43 43 47 41 43 |GTCCAACC|AAACCGAC| |00000b40| 54 43 54 47 41 43 47 47 | 43 41 47 54 54 54 41 43 |TCTGACGG|CAGTTTAC| |00000b50| 47 41 47 41 47 41 47 41 | 54 47 41 54 41 47 47 47 |GAGAGAGA|TGATAGGG| |00000b60| 54 43 54 47 43 54 54 43 | 41 47 54 41 41 47 43 43 |TCTGCTTC|AGTAAGCC| |00000b70| 41 47 41 54 47 43 54 41 | 43 41 43 41 41 54 54 41 |AGATGCTA|CACAATTA| |00000b80| 47 47 43 54 54 47 54 41 | 43 41 54 41 54 54 47 54 |GGCTTGTA|CATATTGT| |00000b90| 43 47 54 54 41 47 41 41 | 43 47 43 47 47 43 54 41 |CGTTAGAA|CGCGGCTA| |00000ba0| 43 41 41 54 54 41 41 54 | 41 43 41 54 41 41 43 43 |CAATTAAT|ACATAACC| |00000bb0| 54 54 41 54 47 54 41 54 | 43 41 54 41 43 41 43 41 |TTATGTAT|CATACACA| |00000bc0| 54 41 43 47 41 54 54 54 | 41 47 47 54 47 41 43 41 |TACGATTT|AGGTGACA| |00000bd0| 43 54 41 54 41 00 00 01 | 0e 00 00 0b 35 00 06 00 |CTATA...|....5...| |00000be0| 00 00 00 00 00 00 09 00 | 01 00 04 00 01 00 09 dd |........|........| |00000bf0| 6b 08 c2 06 a2 01 f4 ff | ff ff ff ff ff ff ff 00 |k.......|........| |00000c00| 07 00 00 00 10 00 10 00 | 01 00 00 00 00 00 09 00 |........|........| |00000c10| 00 00 3c 00 01 00 04 00 | 01 00 09 00 00 00 00 d4 |..<.....|........| |00000c20| 00 01 28 cc cc cc cc cc | cc cc cc 00 09 00 00 00 |..(.....|........| |00000c30| 10 00 10 00 01 00 00 00 | 00 00 3c 00 00 00 43 00 |........|..<...C.| |00000c40| 01 00 04 00 01 00 09 dd | 6b 08 c2 06 a2 01 f4 ff |........|k.......| |00000c50| ff ff ff ff ff ff ff 00 | 07 00 00 00 10 00 10 00 |........|........| |00000c60| 01 00 00 00 00 00 43 00 | 00 00 57 00 01 00 04 00 |......C.|..W.....| |00000c70| 01 00 09 00 00 80 00 11 | b0 01 68 aa 55 aa 55 aa |........|..h.U.U.| |00000c80| 55 aa 55 00 07 00 00 00 | 10 00 10 00 01 00 00 00 |U.U.....|........| |00000c90| 00 00 57 00 00 0b 22 00 | 01 00 04 00 01 00 09 dd |..W...".|........| |00000ca0| 6b 08 c2 06 a2 01 f4 ff | ff ff ff ff ff ff ff 00 |k.......|........| |00000cb0| 07 00 00 00 10 00 10 00 | 01 00 00 00 00 0b 22 00 |........|......".| |00000cc0| 00 0b 35 00 01 00 04 00 | 01 00 09 00 00 80 00 11 |..5.....|........| |00000cd0| b0 01 f8 aa 55 aa 55 aa | 55 aa 55 00 07 00 00 00 |....U.U.|U.U.....| |00000ce0| 10 00 10 00 01 00 00 00 | 00 00 ce 00 00 0b 35 00 |........|......5.| |00000cf0| 04 00 00 00 09 00 00 00 | 3c 00 1a 00 00 00 0c 00 |........|<.......| |00000d00| 00 00 00 00 01 00 42 00 | 08 00 0a 00 04 00 32 00 |......B.|......2.| |00000d10| 09 00 00 00 00 00 00 00 | 58 18 b4 00 00 00 00 00 |........|X.......| |00000d20| 00 00 00 00 00 00 43 00 | 00 00 57 00 2b 00 00 00 |......C.|..W.+...| |00000d30| 0c 00 00 00 00 00 01 00 | 57 00 08 00 0a 00 04 00 |........|W.......| |00000d40| 42 00 09 00 00 00 00 00 | 00 00 58 15 78 00 58 15 |B.......|..X.x.X.| |00000d50| dc 01 00 00 00 00 00 04 | 4c 00 00 07 61 00 1a 00 |........|L...a...| |00000d60| 00 00 0c 00 58 15 d4 00 | 01 01 42 00 08 00 0a 00 |....X...|..B.....| |00000d70| 04 00 32 00 09 02 41 ab | 54 ea ff 00 58 13 f8 00 |..2...A.|T...X...| |00000d80| 58 15 b0 00 00 00 00 00 | 00 0b 22 00 00 0b 35 00 |X.......|.."...5.| |00000d90| e7 00 00 00 0c 00 00 00 | 00 00 01 00 35 00 08 00 |........|....5...| |00000da0| 0a 00 04 00 42 00 09 00 | 00 00 00 00 00 00 58 15 |....B...|......X.| |00000db0| 94 00 58 16 98 01 00 00 | 00 12 70 6f 6c 79 63 6c |..X.....|..polycl| |00000dc0| 6f 6e 69 6e 67 20 72 65 | 67 69 6f 6e 0b 54 37 20 |oning re|gion.T7 | |00000dd0| 70 72 6f 6d 6f 74 65 72 | 00 00 00 11 63 6f 75 6e |promoter|....coun| |00000de0| 74 65 72 2d 63 6c 6f 63 | 6b 77 69 73 65 15 41 6d |ter-cloc|kwise.Am| |00000df0| 70 69 63 69 6c 6c 69 6e | 20 72 65 73 69 73 74 61 |picillin| resista| |00000e00| 6e 63 65 00 00 00 82 a7 | 2d 6c 61 63 74 61 6d 61 |nce.....|-lactama| |00000e10| 73 65 2e 20 20 4e 6f 74 | 65 20 74 68 61 74 20 74 |se. 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P(h.|8$T j.& | |00001310| 50 22 68 00 08 22 51 30 | 2e 00 0c 48 c0 2f 00 2f |P"h.."Q0|...H././| |00001320| 3c 00 00 00 32 4e ad 02 | e2 20 1f 22 71 08 ee 22 |<...2N..|. ."q.."| |00001330| 51 41 ee ff 00 70 3f 20 | d9 51 c8 ff fc 7e 00 20 |QA...p? |.Q...~. | |00001340| 6a 01 26 20 50 2c 28 00 | 02 2f 07 2f 06 48 7a fe |j.& P,(.|././.Hz.| |00001350| c8 2f 0e 2f 0c 4e ad 2c | da 4c df 1c c0 4e 5e 20 |././.N.,|.L...N^ | |00001360| 5f 5c 4f 4e d0 00 00 4e | 56 ff f8 48 e7 03 38 2c |_\ON...N|V..H..8,| |00001370| 2e 00 08 28 46 20 54 26 | 68 00 0c 24 53 43 ea 00 |...(F T&|h..$SC..| |00001380| 38 22 51 2d 49 ff f8 70 | 02 b0 2e 00 1a 66 0a 2d |8"Q-I..p|.....f.-| |00001390| 6d f9 68 00 1c 60 00 01 | 52 4a 2e 00 1a 66 00 01 |m.h..`..|RJ...f..| |000013a0| 46 42 a7 05 70 47 45 4d | 32 00 00 0a 6d 54 68 69 |FB..pGEM|2...mThi| |000013b0| 73 20 66 69 6c 65 20 77 | 61 73 20 64 6f 77 6e 6c |s file w|as downl| |000013c0| 6f 61 64 65 64 20 66 72 | 6f 6d 20 74 68 65 20 31 |oaded fr|om the 1| |000013d0| 32 2f 38 37 20 56 65 63 | 42 61 73 65 20 62 79 20 |2/87 Vec|Base by | |000013e0| 4b 65 69 74 68 20 41 20 | 4a 6f 68 6e 73 6f 6e 20 |Keith A |Johnson | |000013f0| 6f 6e 20 44 65 63 2e 20 | 31 34 2c 20 31 39 38 39 |on Dec. |14, 1989| |00001400| 2e 0d 4d 6f 64 69 66 69 | 65 64 20 62 79 20 42 6f |..Modifi|ed by Bo| |00001410| 62 20 47 72 6f 73 73 2c | 20 31 32 2f 32 36 2f 38 |b Gross,| 12/26/8| |00001420| 39 2e 0d 0d 45 4e 54 52 | 59 20 50 47 45 4d 32 20 |9...ENTR|Y PGEM2 | |00001430| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 | | | |00001440| 20 20 20 23 54 59 50 45 | 20 44 4e 41 20 43 49 52 | #TYPE| DNA CIR| |00001450| 43 55 4c 41 52 0d 54 49 | 54 4c 45 20 70 47 45 4d |CULAR.TI|TLE pGEM| |00001460| 32 20 2d 20 43 6c 6f 6e | 69 6e 67 20 56 65 63 74 |2 - Clon|ing Vect| |00001470| 6f 72 20 47 65 6d 69 6e | 69 20 32 20 66 72 6f 6d |or Gemin|i 2 from| |00001480| 20 50 72 6f 6d 65 67 61 | 20 42 69 6f 74 65 63 0d | Promega| Biotec.| |00001490| 44 41 54 45 20 20 20 20 | 20 20 32 37 2d 4d 41 52 |DATE | 27-MAR| |000014a0| 2d 31 39 38 36 0d 20 20 | 20 23 73 65 71 75 65 6e |-1986. | #sequen| |000014b0| 63 65 20 20 31 31 2d 44 | 45 43 2d 31 39 38 36 0d |ce 11-D|EC-1986.| |000014c0| 20 20 20 23 73 65 71 75 | 65 6e 63 65 20 20 31 36 | #sequ|ence 16| |000014d0| 2d 44 45 43 2d 31 39 38 | 36 0d 41 43 43 45 53 53 |-DEC-198|6.ACCESS| |000014e0| 49 4f 4e 20 56 42 30 30 | 33 37 0d 53 4f 55 52 43 |ION VB00|37.SOURC| |000014f0| 45 20 20 20 20 61 72 74 | 69 66 69 63 69 61 6c 0d |E art|ificial.| |00001500| 52 45 46 45 52 45 4e 43 | 45 0d 20 20 20 23 6e 75 |REFERENC|E. #nu| |00001510| 6d 62 65 72 20 31 0d 20 | 20 20 23 63 69 74 61 74 |mber 1. | #citat| |00001520| 69 6f 6e 20 20 50 72 6f | 6d 65 67 61 20 74 65 63 |ion Pro|mega tec| |00001530| 68 6e 69 63 61 6c 20 42 | 75 6c 6c 65 74 69 6e 0d |hnical B|ulletin.| |00001540| 20 20 20 23 63 6f 6d 6d | 65 6e 74 20 20 20 54 68 | #comm|ent Th| |00001550| 65 20 73 65 71 75 65 6e | 63 65 20 77 61 73 20 72 |e sequen|ce was r| |00001560| 65 76 69 73 65 64 20 62 | 79 20 50 72 6f 6d 65 67 |evised b|y Promeg| |00001570| 61 20 42 69 6f 74 65 63 | 20 4a 55 4c 2d 31 39 38 |a Biotec| JUL-198| |00001580| 36 0d 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |6. | | |00001590| 50 72 6f 6d 65 67 61 20 | 61 6c 73 6f 20 63 6f 6e |Promega |also con| |000015a0| 66 69 72 6d 65 64 20 74 | 68 65 20 72 65 76 69 73 |firmed t|he revis| |000015b0| 69 6f 6e 20 6f 66 20 44 | 45 43 2d 31 39 38 36 0d |ion of D|EC-1986.| |000015c0| 20 20 20 23 63 6f 6d 6d | 65 6e 74 20 20 63 6f 70 | #comm|ent cop| |000015d0| 79 72 69 67 68 74 20 6f | 66 20 74 68 65 20 73 65 |yright o|f the se| |000015e0| 71 75 65 6e 63 65 20 62 | 79 20 50 72 6f 6d 65 67 |quence b|y Promeg| |000015f0| 61 0d 43 4f 4d 4d 45 4e | 54 0d 20 20 20 45 6e 74 |a.COMMEN|T. Ent| |00001600| 65 72 65 64 20 62 79 20 | 57 69 6c 6c 69 61 6d 20 |ered by |William | |00001610| 47 69 6c 62 65 72 74 2c | 20 57 68 69 74 61 6b 65 |Gilbert,| Whitake| |00001620| 72 20 43 6f 6c 6c 65 67 | 65 2c 20 4d 49 54 0d 20 |r Colleg|e, MIT. | |00001630| 20 20 20 20 20 20 61 6e | 64 20 62 79 20 4a 2e 20 | an|d by J. | |00001640| 53 6f 70 70 61 2c 20 61 | 6e 64 20 46 2e 20 50 66 |Soppa, a|nd F. Pf| |00001650| 65 69 66 66 65 72 2c 20 | 4d 50 49 2c 20 4d 61 72 |eiffer, |MPI, Mar| |00001660| 74 69 6e 73 72 69 65 64 | 0d 20 20 20 52 65 76 69 |tinsried|. Revi| |00001670| 73 65 64 20 31 31 2d 44 | 45 43 2d 31 39 38 36 20 |sed 11-D|EC-1986 | |00001680| 62 79 20 46 2e 20 50 66 | 65 69 66 66 65 72 20 61 |by F. Pf|eiffer a| |00001690| 63 63 6f 72 64 69 6e 67 | 20 74 6f 20 50 72 6f 6d |ccording| to Prom| |000016a0| 65 67 61 0d 20 20 20 52 | 65 76 69 73 65 64 20 31 |ega. R|evised 1| |000016b0| 36 2d 44 45 43 2d 31 39 | 38 36 20 62 79 20 46 2e |6-DEC-19|86 by F.| |000016c0| 20 50 66 65 69 66 66 65 | 72 3a 0d 20 20 20 35 33 | Pfeiffe|r:. 53| |000016d0| 34 2f 35 20 22 41 54 22 | 20 74 6f 20 22 54 41 22 |4/5 "AT"| to "TA"| |000016e0| 20 74 6f 20 6d 61 74 63 | 68 20 72 65 76 69 73 65 | to matc|h revise| |000016f0| 64 20 73 65 71 75 65 6e | 63 65 20 6f 66 20 50 42 |d sequen|ce of PB| |00001700| 52 33 32 32 0d 43 4f 4d | 4d 45 4e 54 0d 20 20 20 |R322.COM|MENT. | |00001710| 54 68 65 20 70 47 45 4d | 32 20 76 65 63 74 6f 72 |The pGEM|2 vector| |00001720| 20 63 6f 6e 74 61 69 6e | 73 20 62 6f 74 68 20 74 | contain|s both t| |00001730| 68 65 20 54 37 20 61 6e | 64 20 53 50 36 20 52 4e |he T7 an|d SP6 RN| |00001740| 41 20 70 6f 6c 79 6d 65 | 72 61 73 65 20 70 72 6f |A polyme|rase pro| |00001750| 6d 6f 74 65 72 73 0d 20 | 20 20 73 65 70 65 72 61 |moters. | sepera| |00001760| 74 65 64 20 62 79 20 61 | 20 73 68 6f 72 74 20 70 |ted by a| short p| |00001770| 69 65 63 65 20 6f 66 20 | 44 4e 41 20 63 6f 6e 74 |iece of |DNA cont| |00001780| 61 69 6e 69 6e 67 20 31 | 31 20 75 6e 69 71 75 65 |aining 1|1 unique| |00001790| 20 72 65 73 74 72 69 63 | 74 69 6f 6e 20 73 69 74 | restric|tion sit| |000017a0| 65 73 2e 0d 20 20 20 49 | 74 20 77 61 73 20 63 6f |es.. I|t was co| |000017b0| 6e 73 74 72 75 63 74 65 | 64 20 66 72 6f 6d 20 70 |nstructe|d from p| |000017c0| 53 50 36 35 20 61 6e 64 | 20 61 20 62 61 63 74 65 |SP65 and| a bacte| |000017d0| 72 69 6f 70 68 61 67 65 | 20 54 37 20 70 72 6f 6d |riophage| T7 prom| |000017e0| 6f 74 65 72 2e 0d 20 20 | 20 44 75 72 69 6e 67 20 |oter.. | During | |000017f0| 63 6f 6e 73 74 72 75 63 | 74 69 6f 6e 20 6f 66 20 |construc|tion of | |00001800| 74 68 65 20 53 50 36 20 | 76 65 63 74 6f 72 73 2c |the SP6 |vectors,| |00001810| 20 74 68 65 20 6d 61 6a | 6f 72 69 74 79 20 6f 66 | the maj|ority of| |00001820| 20 74 68 65 20 6c 61 63 | 5a 20 67 65 6e 65 0d 20 | the lac|Z gene. | |00001830| 20 20 77 61 73 20 64 65 | 6c 65 74 65 64 2e 20 20 | was de|leted. | |00001840| 54 68 65 72 65 66 6f 72 | 65 2c 20 74 68 65 20 58 |Therefor|e, the X| |00001850| 2d 67 61 6c 20 63 6f 6c | 6f 72 20 73 63 72 65 65 |-gal col|or scree| |00001860| 6e 69 6e 67 20 6d 65 74 | 68 6f 64 20 66 6f 72 20 |ning met|hod for | |00001870| 74 68 65 0d 20 20 20 69 | 64 65 6e 74 69 66 69 63 |the. i|dentific| |00001880| 61 74 69 6f 6e 20 6f 66 | 20 72 65 63 6f 6d 62 69 |ation of| recombi| |00001890| 6e 61 6e 74 73 20 69 73 | 20 6e 6f 74 20 70 6f 73 |nants is| not pos| |000018a0| 73 69 62 6c 65 20 77 69 | 74 68 20 74 68 69 73 20 |sible wi|th this | |000018b0| 76 65 63 74 6f 72 2e 0d | 4b 45 59 57 4f 52 44 53 |vector..|KEYWORDS| |000018c0| 0d 43 52 4f 53 53 52 45 | 46 45 52 45 4e 43 45 0d |.CROSSRE|FERENCE.| |000018d0| 20 20 20 23 70 61 72 65 | 6e 74 0d 20 20 20 20 20 | #pare|nt. | |000018e0| 56 65 63 42 61 73 65 28 | 33 29 3a 70 53 50 36 35 |VecBase(|3):pSP65| |000018f0| 0d 20 20 20 23 70 61 72 | 65 6e 74 0d 20 20 20 20 |. #par|ent. | |00001900| 20 56 65 63 42 61 73 65 | 28 33 29 3a 70 55 43 31 | VecBase|(3):pUC1| |00001910| 33 2c 20 56 65 63 53 6f | 75 72 63 65 28 33 29 3a |3, VecSo|urce(3):| |00001920| 62 47 61 6c 31 33 2c 0d | 20 20 20 20 20 56 65 63 |bGal13,.| Vec| |00001930| 53 6f 75 72 63 65 28 33 | 29 3a 50 72 6f 6d 53 50 |Source(3|):PromSP| |00001940| 36 2c 20 56 65 63 53 6f | 75 72 63 65 28 33 29 3a |6, VecSo|urce(3):| |00001950| 50 72 6f 6d 54 37 2c 0d | 20 20 20 20 20 56 65 63 |PromT7,.| Vec| |00001960| 42 61 73 65 28 33 29 3a | 70 42 52 33 32 32 0d 20 |Base(3):|pBR322. | |00001970| 20 20 23 62 72 6f 74 68 | 65 72 0d 20 20 20 20 20 | #broth|er. | |00001980| 56 65 63 42 61 73 65 28 | 33 29 3a 70 47 45 4d 31 |VecBase(|3):pGEM1| |00001990| 0d 20 20 20 23 6f 66 66 | 73 70 72 69 6e 67 0d 20 |. #off|spring. | |000019a0| 20 20 20 20 56 65 63 42 | 61 73 65 28 33 29 3a 70 | VecB|ase(3):p| |000019b0| 47 45 4d 34 0d 50 41 52 | 45 4e 54 0d 20 20 20 46 |GEM4.PAR|ENT. F| |000019c0| 65 61 74 75 72 65 73 20 | 6f 66 20 70 47 45 4d 32 |eatures |of pGEM2| |000019d0| 20 28 32 38 36 39 20 62 | 70 29 0d 20 20 20 20 20 | (2869 b|p). | |000019e0| 72 65 73 69 64 75 65 20 | 20 20 20 20 73 6f 75 72 |residue | sour| |000019f0| 63 65 0d 20 20 20 20 20 | 31 30 2d 20 20 36 30 20 |ce. |10- 60 | |00001a00| 20 20 20 35 31 2d 20 20 | 20 31 20 28 63 29 20 4d | 51- | 1 (c) M| |00001a10| 31 33 6d 70 31 31 2f 70 | 55 43 31 33 2d 70 6f 6c |13mp11/p|UC13-pol| |00001a20| 79 6c 69 6e 6b 65 72 0d | 20 20 20 20 20 36 38 2d |ylinker.| 68-| |00001a30| 20 20 38 37 20 20 20 20 | 32 30 2d 20 20 20 31 20 | 87 |20- 1 | |00001a40| 28 63 29 20 54 37 20 70 | 72 6f 6d 6f 74 65 72 0d |(c) T7 p|romoter.| |00001a50| 20 20 20 20 20 39 38 2d | 20 31 35 36 20 20 20 20 | 98-| 156 | |00001a60| 35 39 2d 20 20 20 31 20 | 28 63 29 20 70 55 43 31 |59- 1 |(c) pUC1| |00001a70| 33 0d 20 20 20 20 31 35 | 37 2d 32 33 34 35 20 20 |3. 15|7-2345 | |00001a80| 32 36 38 30 2d 20 34 39 | 32 20 28 63 29 20 70 55 |2680- 49|2 (c) pU| |00001a90| 43 31 33 0d 20 20 20 32 | 33 34 36 2d 32 36 32 34 |C13. 2|346-2624| |00001aa0| 20 20 20 36 35 34 2d 20 | 33 37 36 20 28 63 29 20 | 654- |376 (c) | |00001ab0| 70 42 52 33 32 32 0d 20 | 20 20 32 36 32 35 2d 32 |pBR322. | 2625-2| |00001ac0| 38 36 39 20 20 78 78 78 | 78 2d 78 78 78 78 20 20 |869 xxx|x-xxxx | |00001ad0| 20 20 20 70 68 61 67 65 | 20 53 50 36 0d 20 20 20 | phage| SP6. | |00001ae0| 32 38 35 31 2d 32 38 36 | 39 20 20 20 20 20 31 2d |2851-286|9 1-| |00001af0| 20 20 31 39 20 20 20 20 | 20 53 50 36 20 70 72 6f | 19 | SP6 pro| |00001b00| 6d 6f 74 65 72 0d 20 20 | 20 43 6f 6e 66 6c 69 63 |moter. | Conflic| |00001b10| 74 20 28 63 66 6c 29 20 | 61 6e 64 20 4d 75 74 61 |t (cfl) |and Muta| |00001b20| 74 69 6f 6e 73 20 28 6d | 75 74 29 3a 20 6e 6f 6e |tions (m|ut): non| |00001b30| 65 0d 50 41 52 45 4e 54 | 0d 20 20 20 46 65 61 74 |e.PARENT|. Feat| |00001b40| 75 72 65 73 20 6f 66 20 | 70 47 45 4d 32 20 28 32 |ures of |pGEM2 (2| |00001b50| 38 36 39 20 62 70 29 0d | 20 20 20 20 20 72 65 73 |869 bp).| res| |00001b60| 69 64 75 65 20 20 20 20 | 20 73 6f 75 72 63 65 0d |idue | source.| |00001b70| 20 20 20 20 20 31 30 2d | 20 20 36 30 20 20 20 20 | 10-| 60 | |00001b80| 35 31 2d 20 20 20 31 20 | 28 63 29 20 4d 31 33 6d |51- 1 |(c) M13m| |00001b90| 70 31 31 2f 70 55 43 31 | 33 2d 70 6f 6c 79 6c 69 |p11/pUC1|3-polyli| |00001ba0| 6e 6b 65 72 0d 20 20 20 | 20 20 36 38 2d 20 20 38 |nker. | 68- 8| |00001bb0| 37 20 20 20 20 32 30 2d | 20 20 20 31 20 28 63 29 |7 20-| 1 (c)| |00001bc0| 20 54 37 20 70 72 6f 6d | 6f 74 65 72 0d 20 20 20 | T7 prom|oter. | |00001bd0| 20 20 39 38 2d 32 31 35 | 38 20 20 20 36 32 30 2d | 98-215|8 620-| |00001be0| 32 36 38 30 20 20 20 20 | 20 70 55 43 31 33 63 0d |2680 | pUC13c.| |00001bf0| 20 20 20 32 31 35 39 2d | 32 33 34 35 20 20 20 20 | 2159-|2345 | |00001c00| 20 31 2d 20 31 38 37 20 | 20 20 20 20 70 55 43 31 | 1- 187 | pUC1| |00001c10| 33 63 0d 20 20 20 32 33 | 34 36 2d 32 36 32 34 20 |3c. 23|46-2624 | |00001c20| 20 20 36 35 34 2d 20 33 | 37 36 20 28 63 29 20 70 | 654- 3|76 (c) p| |00001c30| 42 52 33 32 32 0d 20 20 | 20 32 36 32 35 2d 32 38 |BR322. | 2625-28| |00001c40| 36 39 20 20 78 78 78 78 | 2d 78 78 78 78 20 20 20 |69 xxxx|-xxxx | |00001c50| 20 20 70 68 61 67 65 20 | 53 50 36 0d 20 20 20 32 | phage |SP6. 2| |00001c60| 38 35 31 2d 32 38 36 39 | 20 20 20 20 20 31 2d 20 |851-2869| 1- | |00001c70| 20 31 39 20 20 20 20 20 | 53 50 36 20 70 72 6f 6d | 19 |SP6 prom| |00001c80| 6f 74 65 72 0d 20 20 20 | 43 6f 6e 66 6c 69 63 74 |oter. |Conflict| |00001c90| 20 28 63 66 6c 29 20 61 | 6e 64 20 4d 75 74 61 74 | (cfl) a|nd Mutat| |00001ca0| 69 6f 6e 73 20 28 6d 75 | 74 29 3a 20 6e 6f 6e 65 |ions (mu|t): none| |00001cb0| 0d 46 45 41 54 55 52 45 | 0d 20 20 20 20 20 20 20 |.FEATURE|. | |00001cc0| 20 20 20 20 31 20 20 20 | 20 20 20 20 20 20 20 20 | 1 | | |00001cd0| 73 74 61 72 74 20 6f 66 | 20 53 50 36 2d 52 4e 41 |start of| SP6-RNA| |00001ce0| 20 73 79 6e 74 68 65 73 | 69 73 0d 20 20 20 20 20 | synthes|is. | |00001cf0| 20 20 20 20 20 36 39 20 | 20 20 20 20 20 20 28 63 | 69 | (c| |00001d00| 29 20 73 74 61 72 74 20 | 6f 66 20 54 37 2d 52 4e |) start |of T7-RN| |00001d10| 41 20 73 79 6e 74 68 65 | 73 69 73 0d 20 20 20 31 |A synthe|sis. 1| |00001d20| 31 30 31 2d 31 38 38 39 | 20 37 38 39 2d 31 20 28 |101-1889| 789-1 (| |00001d30| 63 29 20 41 70 2d 52 3b | 20 62 2d 6c 61 63 74 61 |c) Ap-R;| b-lacta| |00001d40| 6d 61 73 65 0d 50 4f 4c | 59 4c 49 4e 4b 45 52 20 |mase.POL|YLINKER | |00001d50| 20 48 69 6e 64 49 49 49 | 2d 50 73 74 49 2d 53 61 | HindIII|-PstI-Sa| |00001d60| 6c 49 2d 58 62 61 49 2d | 42 61 6d 48 49 2d 53 6d |lI-XbaI-|BamHI-Sm| |00001d70| 61 49 2d 53 61 63 49 2d | 45 63 6f 52 49 0d 53 45 |aI-SacI-|EcoRI.SE| |00001d80| 4c 45 43 54 49 4f 4e 20 | 23 72 65 73 69 73 74 61 |LECTION |#resista| |00001d90| 6e 63 65 20 20 41 70 0d | 53 55 4d 4d 41 52 59 20 |nce Ap.|SUMMARY | |00001da0| 20 70 47 45 4d 32 20 20 | 20 20 23 6c 65 6e 67 74 | pGEM2 | #lengt| |00001db0| 68 20 32 38 36 39 20 20 | 20 23 63 68 65 63 6b 73 |h 2869 | #checks| |00001dc0| 75 6d 20 31 35 39 37 0d | 53 45 51 55 45 4e 43 45 |um 1597.|SEQUENCE| |00001dd0| 0d 0d 20 56 65 63 62 61 | 73 65 3a 50 67 65 6d 32 |.. Vecba|se:Pgem2| |00001de0| 20 20 4c 65 6e 67 74 68 | 3a 20 32 38 36 39 20 20 | Length|: 2869 | |00001df0| 44 65 63 65 6d 62 65 72 | 20 31 31 2c 20 31 39 38 |December| 11, 198| |00001e00| 39 20 20 31 30 3a 30 34 | 20 20 43 68 65 63 6b 3a |9 10:04| Check:| |00001e10| 20 31 35 39 37 20 20 2e | 2e 00 00 00 00 00 00 00 | 1597 .|........| |00001e20| 00 00 00 00 00 01 01 01 | 01 00 00 01 00 09 00 2d |........|.......-| |00001e30| 00 10 00 10 01 28 cc cc | cc cc cc cc cc cc dd 6b |.....(..|.......k| |00001e40| 08 c2 06 a2 00 01 01 00 | 00 08 00 0a 00 04 00 1d |........|........| |00001e50| 00 09 02 41 ab 54 ea ff | 00 03 00 c8 00 09 00 11 |...A.T..|........| |00001e60| 00 00 00 00 00 00 00 04 | 00 2d 00 09 00 80 00 00 |........|.-......| |00001e70| 00 00 00 00 3f 80 00 00 | 00 00 00 e6 00 00 01 1e |....?...|........| |00001e80| 00 00 01 02 00 00 01 42 | 00 01 00 fc 00 09 00 00 |.......B|........| |00001e90| 00 00 00 00 00 be 00 80 | 00 00 00 00 06 31 37 36 |........|.....176| |00001ea0| 2e 31 30 ad 3f b2 2d 46 | 00 1c 60 04 2d 46 00 1c |.10.?.-F|..`.-F..| |00001eb0| 4c df 1c c0 4e 5e 20 5f | de fc 00 14 4e d0 00 00 |L...N^ _|....N...| |00001ec0| 00 00 00 04 00 01 2d 74 | ff ff 00 2c 00 00 00 2c |......-t|...,...,| |00001ed0| 00 00 2b 98 21 08 40 80 | 40 80 76 2a 00 5e 09 40 |..+.!.@.|@.v*.^.@| |00001ee0| 40 81 2d 64 00 6e 00 01 | 00 6c 63 56 00 6d f0 ac |@.-d.n..|.lcV.m..| |00001ef0| 40 81 27 a8 40 81 27 6e | 00 6e 00 01 00 6c 63 56 |@.'.@.'n|.n...lcV| |00001f00| 00 5e 09 40 40 81 2d 64 | 00 6e 00 01 00 6c 63 56 |.^.@@.-d|.n...lcV| |00001f10| 00 6d f0 ac 00 6d 40 81 | 27 a8 40 81 27 6e 00 6e |.m...m@.|'.@.'n.n| |00001f20| 00 01 00 6c 63 56 00 6d | f0 ac 00 6d e7 0a 00 6d |...lcV.m|...m...m| |00001f30| 6f 6a 00 5e 85 e0 00 6d | f7 30 00 5e 4c a2 00 6d |oj.^...m|.0.^L..m| |00001f40| 6f fa 00 5e 85 e0 00 5e | 81 3c 00 00 f7 30 00 00 |o..^...^|.<...0..| |00001f50| e3 22 40 80 65 44 00 5e | 40 80 e3 7a 40 80 65 44 |."@.eD.^|@..z@.eD| |00001f60| 00 00 6d 60 00 00 4c a2 | 00 6e 00 01 00 6c 63 56 |..m`..L.|.n...lcV| |00001f70| 00 6e 00 01 00 6c 63 56 | 00 6d 6f fa 00 6d 00 6d |.n...lcV|.mo..m.m| |00001f80| f7 30 00 6d f7 30 00 6d | 00 01 d3 56 00 5e 00 6d |.0.m.0.m|...V.^.m| |00001f90| e5 9e 00 5e 46 ba 00 00 | 01 26 00 6e 00 00 00 00 |...^F...|.&.n....| |00001fa0| 00 00 00 20 00 00 00 20 | 00 04 00 0c 00 00 00 0c |... ... |........| |00001fb0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00001fc0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00001fd0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00001fe0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00001ff0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00002000| 00 00 01 00 00 00 01 9a | 00 00 00 9a 00 00 00 46 |........|.......F| |00002010| 47 54 43 47 54 54 54 47 | 47 54 41 54 47 47 43 54 |GTCGTTTG|GTATGGCT| |00002020| 54 43 41 54 54 43 41 47 | 43 54 43 43 47 47 54 54 |TCATTCAG|CTCCGGTT| |00002030| 05 70 47 45 4d 32 02 00 | 00 00 47 43 4b 63 47 43 |.pGEM2..|..GCKcGC| |00002040| 4b 20 00 00 00 00 00 00 | 00 00 00 00 15 25 00 00 |K ......|.....%..| |00002050| 00 00 47 43 4b 63 47 43 | 4b 20 00 00 00 00 00 00 |..GCKcGC|K ......| |00002060| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00002070| 00 00 ab 83 7a 20 00 00 | 1f 30 00 00 01 e0 54 43 |....z ..|.0....TC| |00002080| 41 47 41 41 47 54 41 41 | 47 54 54 47 47 43 43 47 |AGAAGTAA|GTTGGCCG| |00002090| 43 41 47 54 47 54 54 41 | 54 43 41 43 54 43 41 54 |CAGTGTTA|TCACTCAT| |000020a0| 47 47 54 54 41 54 47 47 | 43 41 47 43 41 43 54 47 |GGTTATGG|CAGCACTG| |000020b0| 43 41 54 41 41 54 54 43 | 54 43 54 54 41 43 54 47 |CATAATTC|TCTTACTG| |000020c0| 54 43 41 54 47 43 43 41 | 54 43 43 47 54 41 41 47 |TCATGCCA|TCCGTAAG| |000020d0| 41 54 47 43 54 54 54 54 | 43 54 47 54 47 41 43 54 |ATGCTTTT|CTGTGACT| |000020e0| 47 47 54 47 41 47 54 41 | 43 54 43 41 41 43 43 41 |GGTGAGTA|CTCAACCA| |000020f0| 41 47 54 43 41 54 54 43 | 54 47 41 47 41 41 54 41 |AGTCATTC|TGAGAATA| |00002100| 00 00 00 78 00 03 00 00 | 00 48 00 50 00 00 00 00 |...x....|.H.P....| |00002110| 02 f0 02 80 ff dc ff ec | 02 f4 02 94 01 02 05 28 |........|.......(| |00002120| 03 fc 00 01 00 00 00 48 | 00 50 00 00 00 00 02 f0 |.......H|.P......| |00002130| 02 80 00 50 00 20 02 80 | 0c 80 00 18 00 01 01 01 |...P. ..|........| |00002140| 00 00 00 01 27 0f 00 01 | 01 01 00 00 00 00 00 00 |....'...|........| |00002150| 00 00 00 00 00 00 00 00 | 00 00 00 01 00 00 00 00 |........|........| |00002160| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00002170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 1a |........|........| |00002180| 01 a2 00 00 00 29 00 00 | 00 03 00 00 01 2b 00 00 |.....)..|.....+..| |00002190| 01 51 00 00 00 00 00 00 | 00 00 00 00 01 00 00 00 |.Q......|........| |000021a0| 01 9a 00 00 00 9a 00 00 | 00 46 00 58 19 50 07 b8 |........|.F.X.P..| |000021b0| 00 00 00 1c 00 46 00 01 | 50 52 4e 54 00 00 00 12 |.....F..|PRNT....| |000021c0| 73 74 61 74 00 00 00 1e | 00 01 ff ff 00 00 00 00 |stat....|........| |000021d0| 00 00 00 00 4b 7f ff ff | 00 00 00 7c 00 58 16 3c |....K...|...|.X.<| |000021e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |000021f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| +--------+-------------------------+-------------------------+--------+--------+